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Product Info Summary
| SKU: | M04041-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Equine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Parvalbumin Monoclonal Antibody
View all Parvalbumin Antibodies
SKU/Catalog Number
M04041-1
Size
100ul
Description
Boster Bio Anti-Parvalbumin Monoclonal Antibody catalog # M04041-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parvalbumin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04041-1)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full-length recombinant human protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04041-1 is reactive to PVALB in Bovine, Equine, Human, Mouse, Pig, Rat
Applications
M04041-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
12.059kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000 ICC/IF or IHC: 1:1,000-1:5,000
Validation Images & Assay Conditions
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Western blot analysis of skeletal muscle lysates and His-tagged human recombinant proteins using mouse mAb to parvalbumin, M04041-1, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse muscle, [3] parvalbumin, [4] calretinin, and [5] calbindin. A band at 12kDa is detected in in muscle lysate and one at 18kDa in the His-tagged recombinant parvalbumin protein lane as expected since the His-tag and other vector derived sequence adds about 6kDa to the molecule. Note that the M04041-1 antibody is not cross-reactive with either calbindin or calretinin despite their related amino acid sequences.
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Immunofluorescent analysis of rat cerebellum section stained with mouse mAb to parvalbumin, M04041-1, dilution 1:1,000, in green, and costained with chicken pAb to calbindin, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 M, and free-floating sections were stained with above antibodies. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear are green.
Protein Target Info & Infographic
Gene/Protein Information For PVALB (Source: Uniprot.org, NCBI)
Gene Name
PVALB
Full Name
Parvalbumin alpha
Weight
12.059kDa
Superfamily
parvalbumin family
Alternative Names
D22S749; MGC116759; parvalbumin alpha; parvalbumin; PV PVALB D22S749 parvalbumin parvalbumin alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PVALB, check out the PVALB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PVALB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Parvalbumin Monoclonal Antibody (M04041-1)
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Customer Reviews
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